Synthetic biology-based diagnostic technologies have improved gold-standard diagnostic methodologies by lowering cost, increasing accuracy, and improving portability. However, there has been little effort in adapting these technologies towards applications related to monitoring the health of plants and crops at the point of use.
Here, we take a step towards this vision by developing an approach that combines isothermal amplification of specific plant DX Pathogen genomic sequences with customizable synthetic RNA regulators that are designed to trigger the production of a colourimetric output in cell-free gene expression reactions. . We demonstrate that our system can detect virus-derived sequences with high sensitivity and specificity, and can be used to detect viruses directly from infected plant material.
In addition, we demonstrate that the entire system can work using only body heat and naked-eye visual analysis of the results. We anticipate that these strategies will be important components of easy-to-use, deployable diagnostic systems that can be configured to detect a variety of important plant pathogens.
Keywords: RNA synthetic biology; cell-free synthetic biology; crop pathogens; isothermal amplification; plant viruses; diagnostics at the point of use.
Application: Isolation of viral RNA
Target: viral RNA
CE certified: Yes, CE-IVD labelled for diagnostic use in the EU and other countries
For US customers, please request equivalent research use only NucleoMag Pathogen, REF 744210.4
Technology: Magnetic Bead Technology
Format: Magnetic beads
Handling: Magnetic separation
Automated use: Yes
Sample material: Respiratory swabs, saliva (human)
Sample amount: ≤ 200 µL swab/saliva wash solution
Fragment size: 300 bp–approx. 50kbp
Typical recovery: Depending on the quantity and quality of the sample
Theoretical binding capacity: 0.4 µg/µL beads
Elution volume: 50–100 µL
Preparation time: 40-120 min/96 preparations, depending on the instruments/automation used
Typical application downstream: NGS, RT−PCR
Storage temperature: 18–25 °C
Shelf Life (from production): 19 Month(s)
Hazardous Material: Yes